hydroxyapatite (hap) type ii ceramic resin Search Results


hydroxyapatite chromatography  (Bio-Rad)
96
Bio-Rad hydroxyapatite chromatography
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
Hydroxyapatite Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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25-ml hydroxyapatite column  (Bio-Rad)
90
Bio-Rad 25-ml hydroxyapatite column
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
25 Ml Hydroxyapatite Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxyapatite-based coatings  (Ceram GmbH)
90
Ceram GmbH hydroxyapatite-based coatings
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
Hydroxyapatite Based Coatings, supplied by Ceram GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxyapatite column  (Bio-Rad)
90
Bio-Rad hydroxyapatite column
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
Hydroxyapatite Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nanosized and microporous precipitated hydroxyapatite  (Ceram GmbH)
90
Ceram GmbH nanosized and microporous precipitated hydroxyapatite
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
Nanosized And Microporous Precipitated Hydroxyapatite, supplied by Ceram GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ha/tcp ceramic powders  (Zimmer Biomet)
90
Zimmer Biomet ha/tcp ceramic powders
MSCT rescued impaired lineage differentiation of BMMSCs in Tsk/+ mice. (A) Toluidine blue staining showing the number of CFU-F from WT control, Tsk/+, and MSCT-treated Tsk/+ BMMSCs. (B) Alizarin red staining of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the capacity to form mineralized nodules when cultured under the osteoinductive conditions. (C) Western blotting analysis of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the expression levels of the osteogenic genes Runx2, ALP, and OCN. β-Actin was used as a protein loading control. (D) Subcutaneous implantation of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs in immunocompromised mice showing that new bone (B) and connective tissue (CT) were generated around <t>the</t> <t>HA/TCP</t> (HA) carrier at 8 weeks post implantation. Scale bar, 50 μm. A semiquantitative analysis shows the amount of bone formation in BMMSC implants. (E) Histological images of distal femurs showing the number of adipocytes in WT, Tsk/+, and MSCT-treated Tsk/+ mouse bone marrow, as assessed by Oil red O staining. Scale bar, 50 μm. (F) The number of Oil red O+ cells in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. (G) The expression levels of adipogenic genes PPARγ and LPL in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. All experimental data were verified in at least three independent experiments. Error bars represent the s.d. from the mean values. ***P< 0.005; **P< 0.001; *P< 0.05.
Ha/Tcp Ceramic Powders, supplied by Zimmer Biomet, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxyapatite ceramics  (Landauer Inc)
90
Landauer Inc hydroxyapatite ceramics
MSCT rescued impaired lineage differentiation of BMMSCs in Tsk/+ mice. (A) Toluidine blue staining showing the number of CFU-F from WT control, Tsk/+, and MSCT-treated Tsk/+ BMMSCs. (B) Alizarin red staining of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the capacity to form mineralized nodules when cultured under the osteoinductive conditions. (C) Western blotting analysis of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the expression levels of the osteogenic genes Runx2, ALP, and OCN. β-Actin was used as a protein loading control. (D) Subcutaneous implantation of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs in immunocompromised mice showing that new bone (B) and connective tissue (CT) were generated around <t>the</t> <t>HA/TCP</t> (HA) carrier at 8 weeks post implantation. Scale bar, 50 μm. A semiquantitative analysis shows the amount of bone formation in BMMSC implants. (E) Histological images of distal femurs showing the number of adipocytes in WT, Tsk/+, and MSCT-treated Tsk/+ mouse bone marrow, as assessed by Oil red O staining. Scale bar, 50 μm. (F) The number of Oil red O+ cells in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. (G) The expression levels of adipogenic genes PPARγ and LPL in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. All experimental data were verified in at least three independent experiments. Error bars represent the s.d. from the mean values. ***P< 0.005; **P< 0.001; *P< 0.05.
Hydroxyapatite Ceramics, supplied by Landauer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxyapatite  (Bio-Rad)
95
Bio-Rad hydroxyapatite
MSCT rescued impaired lineage differentiation of BMMSCs in Tsk/+ mice. (A) Toluidine blue staining showing the number of CFU-F from WT control, Tsk/+, and MSCT-treated Tsk/+ BMMSCs. (B) Alizarin red staining of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the capacity to form mineralized nodules when cultured under the osteoinductive conditions. (C) Western blotting analysis of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the expression levels of the osteogenic genes Runx2, ALP, and OCN. β-Actin was used as a protein loading control. (D) Subcutaneous implantation of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs in immunocompromised mice showing that new bone (B) and connective tissue (CT) were generated around <t>the</t> <t>HA/TCP</t> (HA) carrier at 8 weeks post implantation. Scale bar, 50 μm. A semiquantitative analysis shows the amount of bone formation in BMMSC implants. (E) Histological images of distal femurs showing the number of adipocytes in WT, Tsk/+, and MSCT-treated Tsk/+ mouse bone marrow, as assessed by Oil red O staining. Scale bar, 50 μm. (F) The number of Oil red O+ cells in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. (G) The expression levels of adipogenic genes PPARγ and LPL in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. All experimental data were verified in at least three independent experiments. Error bars represent the s.d. from the mean values. ***P< 0.005; **P< 0.001; *P< 0.05.
Hydroxyapatite, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ha macro.prep  (Bio-Rad)
90
Bio-Rad ha macro.prep
MSCT rescued impaired lineage differentiation of BMMSCs in Tsk/+ mice. (A) Toluidine blue staining showing the number of CFU-F from WT control, Tsk/+, and MSCT-treated Tsk/+ BMMSCs. (B) Alizarin red staining of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the capacity to form mineralized nodules when cultured under the osteoinductive conditions. (C) Western blotting analysis of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the expression levels of the osteogenic genes Runx2, ALP, and OCN. β-Actin was used as a protein loading control. (D) Subcutaneous implantation of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs in immunocompromised mice showing that new bone (B) and connective tissue (CT) were generated around <t>the</t> <t>HA/TCP</t> (HA) carrier at 8 weeks post implantation. Scale bar, 50 μm. A semiquantitative analysis shows the amount of bone formation in BMMSC implants. (E) Histological images of distal femurs showing the number of adipocytes in WT, Tsk/+, and MSCT-treated Tsk/+ mouse bone marrow, as assessed by Oil red O staining. Scale bar, 50 μm. (F) The number of Oil red O+ cells in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. (G) The expression levels of adipogenic genes PPARγ and LPL in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. All experimental data were verified in at least three independent experiments. Error bars represent the s.d. from the mean values. ***P< 0.005; **P< 0.001; *P< 0.05.
Ha Macro.Prep, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxyapatite/tricalcium phosphate (ha/tcp) ceramic powder  (Zimmer Biomet)
90
Zimmer Biomet hydroxyapatite/tricalcium phosphate (ha/tcp) ceramic powder
MSCT rescued impaired lineage differentiation of BMMSCs in Tsk/+ mice. (A) Toluidine blue staining showing the number of CFU-F from WT control, Tsk/+, and MSCT-treated Tsk/+ BMMSCs. (B) Alizarin red staining of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the capacity to form mineralized nodules when cultured under the osteoinductive conditions. (C) Western blotting analysis of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the expression levels of the osteogenic genes Runx2, ALP, and OCN. β-Actin was used as a protein loading control. (D) Subcutaneous implantation of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs in immunocompromised mice showing that new bone (B) and connective tissue (CT) were generated around <t>the</t> <t>HA/TCP</t> (HA) carrier at 8 weeks post implantation. Scale bar, 50 μm. A semiquantitative analysis shows the amount of bone formation in BMMSC implants. (E) Histological images of distal femurs showing the number of adipocytes in WT, Tsk/+, and MSCT-treated Tsk/+ mouse bone marrow, as assessed by Oil red O staining. Scale bar, 50 μm. (F) The number of Oil red O+ cells in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. (G) The expression levels of adipogenic genes PPARγ and LPL in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. All experimental data were verified in at least three independent experiments. Error bars represent the s.d. from the mean values. ***P< 0.005; **P< 0.001; *P< 0.05.
Hydroxyapatite/Tricalcium Phosphate (Ha/Tcp) Ceramic Powder, supplied by Zimmer Biomet, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxyapatite bioceramics  (Ceram GmbH)
90
Ceram GmbH hydroxyapatite bioceramics
MSCT rescued impaired lineage differentiation of BMMSCs in Tsk/+ mice. (A) Toluidine blue staining showing the number of CFU-F from WT control, Tsk/+, and MSCT-treated Tsk/+ BMMSCs. (B) Alizarin red staining of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the capacity to form mineralized nodules when cultured under the osteoinductive conditions. (C) Western blotting analysis of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the expression levels of the osteogenic genes Runx2, ALP, and OCN. β-Actin was used as a protein loading control. (D) Subcutaneous implantation of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs in immunocompromised mice showing that new bone (B) and connective tissue (CT) were generated around <t>the</t> <t>HA/TCP</t> (HA) carrier at 8 weeks post implantation. Scale bar, 50 μm. A semiquantitative analysis shows the amount of bone formation in BMMSC implants. (E) Histological images of distal femurs showing the number of adipocytes in WT, Tsk/+, and MSCT-treated Tsk/+ mouse bone marrow, as assessed by Oil red O staining. Scale bar, 50 μm. (F) The number of Oil red O+ cells in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. (G) The expression levels of adipogenic genes PPARγ and LPL in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. All experimental data were verified in at least three independent experiments. Error bars represent the s.d. from the mean values. ***P< 0.005; **P< 0.001; *P< 0.05.
Hydroxyapatite Bioceramics, supplied by Ceram GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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porous hydroxyapatite monoliths  (Ceram GmbH)
90
Ceram GmbH porous hydroxyapatite monoliths
MSCT rescued impaired lineage differentiation of BMMSCs in Tsk/+ mice. (A) Toluidine blue staining showing the number of CFU-F from WT control, Tsk/+, and MSCT-treated Tsk/+ BMMSCs. (B) Alizarin red staining of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the capacity to form mineralized nodules when cultured under the osteoinductive conditions. (C) Western blotting analysis of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the expression levels of the osteogenic genes Runx2, ALP, and OCN. β-Actin was used as a protein loading control. (D) Subcutaneous implantation of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs in immunocompromised mice showing that new bone (B) and connective tissue (CT) were generated around <t>the</t> <t>HA/TCP</t> (HA) carrier at 8 weeks post implantation. Scale bar, 50 μm. A semiquantitative analysis shows the amount of bone formation in BMMSC implants. (E) Histological images of distal femurs showing the number of adipocytes in WT, Tsk/+, and MSCT-treated Tsk/+ mouse bone marrow, as assessed by Oil red O staining. Scale bar, 50 μm. (F) The number of Oil red O+ cells in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. (G) The expression levels of adipogenic genes PPARγ and LPL in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. All experimental data were verified in at least three independent experiments. Error bars represent the s.d. from the mean values. ***P< 0.005; **P< 0.001; *P< 0.05.
Porous Hydroxyapatite Monoliths, supplied by Ceram GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column chromatography steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic hydroxyapatite Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).

Journal: The Journal of biological chemistry

Article Title: Lipid dependence and basic kinetics of the purified 1,2-diacylglycerol 3-glucosyltransferase from membranes of Acholeplasma laidlawii.

doi: 10.1074/jbc.272.2.929

Figure Lengend Snippet: FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column chromatography steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic hydroxyapatite Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).

Article Snippet: Hydroxyapatite Chromatography (HAC)—A column (16 mm in diameter) packed with 6 ml of ceramic hydroxyapatite Macro-Prep (Bio-Rad) was equilibrated with HAC-buffer: 100 mM KH2PO4/K2HPO4, pH 8, 20% (v/v) glycerol, and 20 mM CHAPS.

Techniques: Purification, Column Chromatography, Activity Assay, Concentration Assay, Filtration

MSCT rescued impaired lineage differentiation of BMMSCs in Tsk/+ mice. (A) Toluidine blue staining showing the number of CFU-F from WT control, Tsk/+, and MSCT-treated Tsk/+ BMMSCs. (B) Alizarin red staining of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the capacity to form mineralized nodules when cultured under the osteoinductive conditions. (C) Western blotting analysis of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the expression levels of the osteogenic genes Runx2, ALP, and OCN. β-Actin was used as a protein loading control. (D) Subcutaneous implantation of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs in immunocompromised mice showing that new bone (B) and connective tissue (CT) were generated around the HA/TCP (HA) carrier at 8 weeks post implantation. Scale bar, 50 μm. A semiquantitative analysis shows the amount of bone formation in BMMSC implants. (E) Histological images of distal femurs showing the number of adipocytes in WT, Tsk/+, and MSCT-treated Tsk/+ mouse bone marrow, as assessed by Oil red O staining. Scale bar, 50 μm. (F) The number of Oil red O+ cells in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. (G) The expression levels of adipogenic genes PPARγ and LPL in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. All experimental data were verified in at least three independent experiments. Error bars represent the s.d. from the mean values. ***P< 0.005; **P< 0.001; *P< 0.05.

Journal: Cell Research

Article Title: Mesenchymal stem cell transplantation in tight-skin mice identifies miR-151-5p as a therapeutic target for systemic sclerosis

doi: 10.1038/cr.2017.11

Figure Lengend Snippet: MSCT rescued impaired lineage differentiation of BMMSCs in Tsk/+ mice. (A) Toluidine blue staining showing the number of CFU-F from WT control, Tsk/+, and MSCT-treated Tsk/+ BMMSCs. (B) Alizarin red staining of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the capacity to form mineralized nodules when cultured under the osteoinductive conditions. (C) Western blotting analysis of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs showing the expression levels of the osteogenic genes Runx2, ALP, and OCN. β-Actin was used as a protein loading control. (D) Subcutaneous implantation of WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs in immunocompromised mice showing that new bone (B) and connective tissue (CT) were generated around the HA/TCP (HA) carrier at 8 weeks post implantation. Scale bar, 50 μm. A semiquantitative analysis shows the amount of bone formation in BMMSC implants. (E) Histological images of distal femurs showing the number of adipocytes in WT, Tsk/+, and MSCT-treated Tsk/+ mouse bone marrow, as assessed by Oil red O staining. Scale bar, 50 μm. (F) The number of Oil red O+ cells in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. (G) The expression levels of adipogenic genes PPARγ and LPL in WT, Tsk/+, and MSCT-treated Tsk/+ BMMSCs under the adipoinductive conditions. All experimental data were verified in at least three independent experiments. Error bars represent the s.d. from the mean values. ***P< 0.005; **P< 0.001; *P< 0.05.

Article Snippet: In vivo BMMSC implantation 4.0 × 10 6 BMMSCs, isolated from C57BL/6J, Tsk / + , MSCT, exosome-treated, and Ad-miR151-treated mice, were mixed with HA/TCP ceramic powders (40 mg, Zimmer Inc.) and implanted into 8-week-old nude mice subcutaneously.

Techniques: Staining, Cell Culture, Western Blot, Expressing, Generated